Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MCM4 Double Nickase Plasmid (h): sc-402451-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCM4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MCM4 Double Nickase Plasmid (h) and MCM4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MCM4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MCM4 Antibody (G-7): sc-28317
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCM4 Double Nickase Plasmid (h) | sc-402451-NIC | 20 µg | $410.00 | |||
MCM4 Double Nickase Plasmid (h2) | sc-402451-NIC-2 | 20 µg | $410.00 |
MCM4 encodes a core subunit of the minichromosome maintenance (MCM2–7) helicase complex that licenses replication origins and drives DNA unwinding during S phase. Through regulation by CDK and DDK signaling, MCM4 participates in replication initiation and elongation, coordinates replication fork progression, and supports genome stability under replication stress. Perturbation of MCM4 function disrupts DNA replication timing and activates checkpoint pathways such as ATR–CHK1, linking MCM4 to mechanisms that safeguard against DNA damage accumulation. Altered expression or dysfunction of MCM4 has been associated with proliferative phenotypes and genomic instability relevant to cancer biology and inherited disorders of DNA replication and repair.
MCM4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MCM4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MCM4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MCM4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MCM4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.