Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Jagged2 Double Nickase Plasmid (h): sc-401322-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Jagged2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Jagged2 Double Nickase Plasmid (h) and Jagged2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting JAG2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Jagged2 Antibody (F-4): sc-515725
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Jagged2 Double Nickase Plasmid (h) | sc-401322-NIC | 20 µg | $410.00 | |||
Jagged2 Double Nickase Plasmid (h2) | sc-401322-NIC-2 | 20 µg | $410.00 |
JAG2 encodes Jagged2, a membrane-bound ligand that engages NOTCH receptors to regulate juxtacrine signaling controlling cell fate decisions, proliferation, and differentiation. Jagged2-mediated NOTCH activation influences transcriptional programs through RBPJ/CSL-dependent target genes and intersects with developmental and tissue homeostasis pathways. Dysregulated JAG2/NOTCH signaling has been implicated in aberrant lineage specification, altered stem or progenitor cell behavior, and context-dependent changes in epithelial–mesenchymal dynamics. As a result, JAG2 is frequently studied in models of development, immunobiology, and diseases characterized by perturbed NOTCH pathway activity.
Jagged2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the JAG2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within JAG2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt JAG2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of JAG2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.