IL-3 Antibody (H-8): sc-515305

IL-3 Antibody (H-8) is a mouse monoclonal IgG2b antibody that detects interleukin-3 (IL-3) in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-3 is a crucial pleiotropic cytokine primarily secreted by activated T lymphocytes, playing a significant role in the proliferation and differentiation of hematopoietic cells. Anti-IL-3 antibody (H-8) supports the growth of pluripotent stem cells and more differentiated progenitors while enhancing the functional activity of fully differentiated cells, making IL-3 vital for maintaining immune homeostasis. The IL-3 receptor, which consists of a ligand-specific α subunit and a common β subunit shared with IL-5 and GM-CSF receptors, is essential for mediating IL-3 biological effects. Notably, the carboxy terminus of the β subunit is critical for activating the MAP kinase signaling pathway, which is pivotal for various cellular responses. Although the IL-3 receptor lacks intrinsic kinase activity, IL-3 stimulation leads to the tyrosine phosphorylation of JAK2, a member of the JAK/Tyk 2 family, subsequently activating Stat5a and Stat5b, which translocate to the nucleus to regulate gene expression. IL-3 monoclonal antibody (H-8) is an invaluable tool for researchers studying hematopoiesis and immune responses, providing insights into the complex signaling pathways involved in these processes.

The preferred IL-3 antibody is IL-3 Antibody (G-1): sc-28342.