Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HXK III Double Nickase Plasmid (h): sc-403846-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HXK III Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HXK III Double Nickase Plasmid (h) and HXK III Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HK3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HXK III Antibody (A-9): sc-74488
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HXK III Double Nickase Plasmid (h) | sc-403846-NIC | 20 µg | $410.00 | |||
HXK III Double Nickase Plasmid (h2) | sc-403846-NIC-2 | 20 µg | $410.00 |
Human HK3 encodes hexokinase 3 (HXK III), an ATP-dependent enzyme that phosphorylates glucose to glucose-6-phosphate, committing glucose to intracellular metabolism and coupling nutrient availability to energy production. By controlling entry into glycolysis and contributing substrates for the pentose phosphate pathway and glycogen synthesis, HXK III helps shape cellular redox balance and biosynthetic capacity. HK3 expression is enriched in hematopoietic and myeloid lineages, linking its activity to immune-cell metabolic programming during activation and differentiation. Dysregulated hexokinase activity and altered glucose utilization are frequently associated with metabolic reprogramming observed in cancer and inflammatory states, making HK3 a useful node for studying disease-relevant bioenergetics.
HXK III Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HK3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HK3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HK3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HK3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.