Hoechst 33342 trihydrochloride CAS: 23491-52-3
MF: C27H28N6O•3HCl
MW: 561.93
An A:T-specific DNA minor groove ligand used for visualizing cellular DNA.

Hoechst 33342 trihydrochloride (CAS 23491-52-3)

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同義語 Bisbenzimide
アプリケーション: An A:T-specific DNA minor groove ligand used for visualizing cellular DNA
CAS 番号: 23491-52-3
Purity: ≥95%
Molecular Weight: 561.93
Molecular Formula: C27H28N6O•3HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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Hoechst 33342 trihydrochloride is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin (sc-200923) has been shown to disrupt the DNA dye update.

The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342's fluorescent capability, the chemical has also had varying effects on differing cell types. The stain has been shown to induce cell differentiation, bind reversibly to Friend erythroleukemia cells, inhibit Topo I (topoisomerase I), induce apoptosis in HL-60 cells, and in junctional sarcoplasmic reticulum cells regulate Ca2+ efflux and affect cell differentiation.


参考文献

1. Latt, S.A., et al. 1976. J. Histochem. Cytochem. 24: 24-33. PMID: 943439
2. Arndt-Jovin, D.J., et al. 1977. J. Histochem. Cytochem. 25: 585-589. PMID: 70450
3. Lydon, M.J., et al. 1980. J. Cell. Physiol. 102: 175-181. PMID: 6154714
4. Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. U.S.A. 78: 363-367. PMID: 6941251
5. Gregoire, M., et al. 1984. Exp. Cell Res. 152: 38-46. PMID: 6201374
6. Crissman, H.A., et al. 1987. Exp. Cell Res. 173: 256-261. PMID: 2960553
7. Loontiens, F.G., et al. 1990. Biochemistry. 29: 9029-9039. PMID: 1702995
8. Steuer, B., et al. 1990. Exp. Cell Res. 186: 149-157. PMID: 2298232
9. Crissman, H.A., et al. 1990. Methods Cell Biol. 33: 97-103. PMID: 1707496
10. Ormerod, M.G., et al. 1993. Cytometry. 14: 595-602. PMID: 8404365
11. Wong, S.S., et al. 1994. Biochem. Pharmacol. 47: 827-837. PMID: 7510966
12. Belloc, F., et al. 1994. Cytometry. 17: 59-65. PMID: 7528124
13. Zhang, X. and Kiechle, F. 2001. Ann. Clin. Lab. Sci. 31: 187-198. PMID: 11337909

Physical State :
Solid
Solubility :
Soluble in water (20 mg/ml). Precipitates in phosphate buffered solutions
保存方法 :
Store at -20° C
Fluoresence :
λex 346 nm, λem 460 nm
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
RTECS :
DT4200000
PubChem CID :
16760503
Merck Index :
14: 10367
MDL Number :
MFCD00012678
SMILES :
CCOC1=CC=C(C=C1)C2=NCC3=C(N2)C=CC(=C3)C4=NCC5=C(N4)C=CC(=C5)N6CCN(CC6)C.Cl.Cl.Cl

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Hoechst 33342 trihydrochloride  参考文献

See how others have used Hoechst 33342 trihydrochloride. Click on the entry to view the PubMed entry .

Citations 1 to 5 of 5 total

PMID: # 26432900  Garbati, MR. et al. 2016. Journal of leukocyte biology. 99: 455-65.

PMID: # 22846576  Luo, Y. et al. 2012. Biochem. Biophys. Res. Commun. 425: 473-7.

PMID: # 31545406  Int. J. Mol. Med. 44: 1677-1686.

PMID: # 31758463  Methods Mol. Biol. 2071: 371-409.

PMID: # 31971826  DNA Cell Biol. 39: 310-320.

Citations 1 to 5 of 5 total
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