HEPES Sodium salt CAS: 75277-39-3
MF: C8H17N2NaO4S
MW: 260.3
A zwitterionic all-purpose buffer.

HEPES Sodium salt (CAS 75277-39-3)

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別名: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
アプリケーション: HEPES Sodium salt is a zwitterionic all-purpose buffer
CAS 番号: 75277-39-3
Purity: ≥99%
Molecular Weight: 260.3
Molecular Formula: C8H17N2NaO4S
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
* Refer to Certificate of Analysis for lot specific data (including water content).
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HEPES Sodium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt) may be used in the preparation of HEPES buffer, which has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.


参考文献

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240
3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745
4. Himmel, H.M., et al. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532
5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256

用法 :
• Recommended working concentration: 10-25 mM

• A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with sodium hydroxide or potassium hydroxide to the pH desired. Alternatively, equimolar concentrations of HEPES (sc-29097) and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH.

• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
Physical State :
Solid
Solubility :
Soluble in water (500 mg/ml).
保存方法 :
Store at room temperature
Refractive Index :
n20D ~1.54 (Predicted)
pK Values :
pKa: 7.5 (25°C)
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
WGK Germany :
3
PubChem CID :
2724248
MDL Number :
MFCD00036463
EC Number :
278-169-7
SMILES :
C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]

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HEPES Sodium salt (CAS 75277-39-3)  参考文献

See how others have used HEPES Sodium salt (CAS 75277-39-3). Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 16531090  Guimarães, ET. et al. 2006. Microbes Infect. 8: 1219-26.

Citations 1 to 1 of 1 total
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