GNL3L Double Nickase Plasmid (h): sc-412308-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GNL3L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GNL3L Double Nickase Plasmid (h) and GNL3L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GNL3L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GNL3L Double Nickase Plasmid (h) | sc-412308-NIC | 20 µg | $410.00 |
GNL3L (guanine nucleotide-binding protein-like 3-like) is a nucleolar GTP-binding protein implicated in ribosome biogenesis and nucleolar organization, with roles in regulating cell-cycle progression and proliferation. It participates in RNA processing and ribonucleoprotein complex assembly, linking nucleolar function to growth control programs. Altered expression of GNL3L has been reported in proliferative contexts and is studied in relation to tumor biology and cellular stress responses that impact nucleolar homeostasis. These features make GNL3L a useful target for investigating how nucleolar GTPases coordinate ribosome production with cell fate decisions.
GNL3L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GNL3L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GNL3L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GNL3L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GNL3L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.