Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GKLF/KLF4 Double Nickase Plasmid (h): sc-400312-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GKLF/KLF4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GKLF/KLF4 Double Nickase Plasmid (h) and GKLF/KLF4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KLF4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GKLF/KLF4 Antibody (B-8): sc-393462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GKLF/KLF4 Double Nickase Plasmid (h) | sc-400312-NIC | 20 µg | $410.00 | |||
GKLF/KLF4 Double Nickase Plasmid (h2) | sc-400312-NIC-2 | 20 µg | $410.00 |
KLF4 (GKLF) encodes a zinc-finger transcription factor that regulates epithelial differentiation, cell-cycle control, and maintenance of cellular identity. By modulating transcriptional programs linked to MAPK, Wnt/β-catenin, and TGF-β signaling, KLF4 influences proliferation, barrier function, and stress responses, including crosstalk with p53-dependent checkpoints. KLF4 is also one of the canonical reprogramming factors and contributes to chromatin-state remodeling during lineage conversion. Dysregulated KLF4 activity has been associated with altered differentiation states and context-dependent roles in oncogenesis and inflammatory pathologies, making it a useful node for mechanistic studies of transcriptional regulation.
GKLF/KLF4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KLF4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KLF4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KLF4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KLF4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.