Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GATA4 Double Nickase Plasmid (h): sc-400122-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GATA4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GATA4 Double Nickase Plasmid (h) and GATA4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GATA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GATA4 Antibody (G-4): sc-25310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GATA4 Double Nickase Plasmid (h) | sc-400122-NIC | 20 µg | $410.00 | |||
GATA4 Double Nickase Plasmid (h2) | sc-400122-NIC-2 | 20 µg | $410.00 |
GATA4 encodes a zinc-finger transcription factor that binds GATA motifs to regulate gene programs controlling cardiac morphogenesis, cardiomyocyte differentiation, and endoderm-derived organ development. In concert with cofactors such as NKX2-5, TBX5, and FOG family proteins, GATA4 integrates signaling inputs including BMP, WNT, and MAPK pathways to coordinate lineage specification and tissue remodeling. Altered GATA4 dosage or sequence can perturb transcriptional networks governing heart and gastrointestinal development, and dysregulation of GATA4-dependent enhancer activity has been linked to congenital heart defects and cardiomyopathy-associated gene expression changes. As a nodal regulator of chromatin-associated transcription, GATA4 is widely used to study developmental gene regulatory circuits and cell fate control.
GATA4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GATA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GATA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GATA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GATA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.