Dynein HC Double Nickase Plasmid (h): sc-400919-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynein HC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dynein HC Double Nickase Plasmid (h) and Dynein HC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DYNC1H1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynein HC Antibody (C-5): sc-514579
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynein HC Double Nickase Plasmid (h) | sc-400919-NIC | 20 µg | $410.00 | |||
Dynein HC Double Nickase Plasmid (h2) | sc-400919-NIC-2 | 20 µg | $410.00 |
DYNC1H1 encodes the cytoplasmic dynein 1 heavy chain, a core ATPase motor that powers minus-end–directed transport along microtubules. Dynein HC coordinates intracellular cargo trafficking, organelle positioning, mitotic spindle assembly, and retrograde axonal transport through interactions with dynactin and cargo adaptors, integrating with microtubule dynamics and cell cycle control pathways. Disruption of dynein-dependent transport has been linked to neurodevelopmental and neuromuscular phenotypes, reflecting the protein’s essential role in neuronal polarity and long-range vesicle movement. Consequently, DYNC1H1 is widely studied in cytoskeletal regulation, neuronal homeostasis, and mechanisms of intracellular logistics.
Dynein HC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DYNC1H1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DYNC1H1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DYNC1H1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DYNC1H1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.