DHCR24 Double Nickase Plasmid (h): sc-405507-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DHCR24 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DHCR24 Double Nickase Plasmid (h) and DHCR24 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DHCR24. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DHCR24 Antibody (A-4): sc-398938
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHCR24 Double Nickase Plasmid (h) | sc-405507-NIC | 20 µg | $410.00 | |||
DHCR24 Double Nickase Plasmid (h2) | sc-405507-NIC-2 | 20 µg | $410.00 |
DHCR24 encodes 24-dehydrocholesterol reductase, an endoplasmic reticulum enzyme that catalyzes the terminal step of cholesterol biosynthesis by reducing desmosterol to cholesterol. Through its role in the Bloch pathway, DHCR24 influences membrane sterol composition, lipid raft organization, and sterol-sensitive signaling networks that intersect with ER homeostasis and oxidative stress responses. Perturbation of DHCR24 activity can shift desmosterol/cholesterol balance, impacting cellular differentiation and metabolic adaptation in sterol-dependent contexts. Altered DHCR24 expression or function has been reported in disorders of cholesterol metabolism and has been studied in neurodegeneration and oncology models for its links to stress resilience and lipid remodeling.
DHCR24 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DHCR24 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DHCR24. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DHCR24 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DHCR24-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.