Date published: 2026-10-23
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Cytokeratin 5/6/18 Antibody (3H2061) - Western Blotting - Image 353708
Cytokeratin 5/6/18 Antibody (3H2061): sc-70926. Western blot analysis of Cytokeratin 18 expression in T24 (A), SK-BR-3 (B), ZR-75-1 (C), PC-3 (D), A-431 (E) and Hep G2 (F) whole cell lysates.
Cytokeratin 5/6/18 Antibody (3H2061): sc-70926. Western blot analysis of Cytokeratin 18 expression in T24 (A), SK-BR-3 (B), ZR-75-1 (C), PC-3 (D), A-431 (E) and Hep G2 (F) whole cell lysates.

Cytokeratin 5/6/18 Antibody (3H2061): sc-70926

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Datasheets
  • Cytokeratin 5/6/18 Antibody (3H2061) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against stratum corneum of human origin
  • recommended for detection of Cytokeratin 5/6/18 of human origin by WB, IP, IF and IHC(P)
  • See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 5/6/18 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Cytokeratin 5/6/18 Antibody (3H2061) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 5/6/18 Antibody (3H2061) (see ordering information below).
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Additional Cytokeratin Antibodies including Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 18, Cytokeratin 19, Cytokeratin 20, Cytokeratin 23, Cytokeratin 2E, Cytokeratin 2p and Cytokeratin 3

Cytokeratin 5/6/18 Antibody (3H2061) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 5/6/18 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Cytokeratin 5/6/18 is a crucial component of the cytoskeletal structure in epithelial cells, playing a significant role in maintaining cellular integrity and providing mechanical support. Anti-Cytokeratin 5/6/18 antibody (3H2061) serves as a reliable marker for identifying specific epithelial cell types and their states, especially in the diagnosis of malignant tumors. Cytokeratin 5 is predominantly expressed in basal cells, while Cytokeratin 6 is found in suprabasal keratinocytes, particularly during wound healing, and Cytokeratin 18 is one of the earliest keratins expressed during embryonic development, persisting in adult simple epithelia and adenocarcinomas. The interaction of these cytokeratins with other proteins, such as desmoplakin and plectin, is vital for the formation of desmosomes and hemidesmosomes, which are essential for cell adhesion and tissue stability. Understanding the expression and interaction of Cytokeratin 5/6/18 can provide insights into various pathological conditions, including epidermolysis bullosa, where mutations in the cytokeratin 5 gene lead to significant skin fragility.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Cytokeratin 5/6/18 Antibody (3H2061) References:

  1. Cytokeratin 5/6 immunostaining in hepatobiliary and pancreatic neoplasms.  |  Vlasoff, DM., et al. 2002. Appl Immunohistochem Mol Morphol. 10: 147-51. PMID: 12051633
  2. The value of calretinin and cytokeratin 5/6 as markers for mesothelioma in cell block preparations of serous effusions.  |  Shield, PW. and Koivurinne, K. 2008. Cytopathology. 19: 218-23. PMID: 17916095
  3. The role of cytokeratin 5/6 as an adjunct diagnostic tool in breast core needle biopsies.  |  Nofech-Mozes, S., et al. 2008. Int J Surg Pathol. 16: 399-406. PMID: 18499686
  4. Usefulness of cytokeratin 5/6 and AMACR applied as double sequential immunostains for diagnostic assessment of problematic prostate specimens.  |  Trpkov, K., et al. 2009. Am J Clin Pathol. 132: 211-20; quiz 307. PMID: 19605815
  5. Biological functions of cytokeratin 18 in cancer.  |  Weng, YR., et al. 2012. Mol Cancer Res. 10: 485-93. PMID: 22452884
  6. The unique luminal staining pattern of cytokeratin 5/6 in adenoid cystic carcinoma of the breast may aid in differentiating it from its mimickers.  |  Nakai, T., et al. 2016. Virchows Arch. 469: 213-22. PMID: 27240462
  7. Nested Variant of Urothelial Carcinoma Is a Luminal Bladder Tumor With Distinct Coexpression of the Basal Marker Cytokeratin 5/6.  |  Johnson, SM., et al. 2021. Am J Clin Pathol. 155: 588-596. PMID: 33118597
  8. Serum and urine periostin and cytokeratin-18 in children with congenital obstructive nephropathy.  |  Turczyn, A., et al. 2022. Cent Eur J Immunol. 47: 63-72. PMID: 35600153
  9. Increase of KLK7, cytokeratin 5/6, and elafin expression in head and neck squamous cell carcinoma compared with lung squamous cell carcinoma.  |  Shanmugam, A., et al. 2024. J Histotechnol. 47: 80-86. PMID: 38189409
  10. Cytokeratin 18 in nonalcoholic fatty liver disease: value and application.  |  Wu, Y., et al. 2024. Expert Rev Mol Diagn. 1-14. PMID: 39387822

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cytokeratin 5/6/18 Antibody (3H2061)

sc-70926
200 µg/ml
$322.00

Cytokeratin 5/6/18 Antibody (3H2061): m-IgG Fc BP-HRP Bundle

sc-551966
200 µg Ab; 10 µg BP
$361.00

Can Cytokeratin 5/6/18 (3H2061): sc-70926 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, Cytokeratin 5/6/18 (3H2061): sc-70926 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by QZ from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by TS from Great Western blot in HeLa samplesGreat Western blot in HeLa samples, detects endogenous Cytokeratin 5/6/18.
Date published: 2015-01-03
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Cytokeratin 5/6/18 Antibody (3H2061) is rated 4.0 out of 5 by 2.
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