Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CTGF CRISPR Activation Plasmid (h): sc-400091-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTGF CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- CTGF CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by CTGF CRISPR Activation Plasmid (h) and CTGF CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CTGF transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CTGF Antibody (E-5): sc-365970
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTGF CRISPR Activation Plasmid (h) | sc-400091-ACT | 20 µg | $397.00 |
Connective tissue growth factor (CTGF/CCN2) is a secreted matricellular protein that integrates extracellular matrix remodeling with growth factor signaling to regulate cell adhesion, migration, proliferation, and fibrotic gene programs. CTGF is inducible by TGF-β and mechanical cues, and it modulates pathways including SMAD signaling, integrin-mediated adhesion, and crosstalk with MAPK and YAP/TAZ mechanotransduction. Through these functions, CTGF shapes tissue repair and stromal remodeling by influencing collagen deposition, myofibroblast activation, and angiogenic responses. Dysregulated CTGF expression is associated with pathological fibrosis and tumor-associated stromal progression, making it a widely used molecular readout and driver in studies of extracellular matrix biology.
CTGF CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CTGF expression without altering the underlying DNA sequence.
CTGF CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CTGF locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CTGF transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous CTGF expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CTGF locus and enabling the study of CTGF-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of CTGF pathway restoration in tumor cells with silenced or reduced CTGF expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.