Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdx2 Double Nickase Plasmid (h): sc-401220-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdx2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdx2 Double Nickase Plasmid (h) and Cdx2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdx2 Antibody (E-1): sc-393572
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdx2 Double Nickase Plasmid (h) | sc-401220-NIC | 20 µg | $410.00 | |||
Cdx2 Double Nickase Plasmid (h2) | sc-401220-NIC-2 | 20 µg | $410.00 |
CDX2 encodes the caudal-type homeobox transcription factor Cdx2, a lineage-determining regulator essential for trophectoderm specification and intestinal epithelial differentiation. Cdx2 binds AT-rich promoter/enhancer elements to control gene programs governing cell polarity, proliferation, and maturation, integrating with Wnt/β-catenin, BMP, and Notch-associated transcriptional networks during gut development and homeostasis. Dysregulated CDX2 expression is commonly used as a molecular feature in gastrointestinal and other epithelial contexts, where altered differentiation states and lineage plasticity are linked to tumor biology and aberrant mucosal remodeling. As a nuclear transcriptional regulator, Cdx2 serves as a tractable node for studying chromatin-dependent control of epithelial identity and stress-adaptive reprogramming.
Cdx2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.