Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CAP1 Double Nickase Plasmid (h): sc-405060-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CAP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CAP1 Double Nickase Plasmid (h) and CAP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CAP1 Antibody (E-4): sc-376191
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAP1 Double Nickase Plasmid (h) | sc-405060-NIC | 20 µg | $410.00 | |||
CAP1 Double Nickase Plasmid (h2) | sc-405060-NIC-2 | 20 µg | $410.00 |
Human CAP1 (cyclase-associated actin cytoskeleton regulatory protein 1) is an actin-binding protein that coordinates actin filament turnover and cytoskeletal remodeling through interactions with cofilin and profilin, supporting cell shape, motility, and endocytic trafficking. CAP1 contributes to regulation of actin dynamics downstream of signaling networks that influence adhesion and migration, linking cytoskeletal state to broader programs such as proliferation and stress responses. Altered CAP1 expression or activity has been associated with changes in cell invasion, epithelial–mesenchymal transition-related phenotypes, and tumor progression in multiple cancer contexts. These functions make CAP1 a useful node for studying actin-dependent processes, membrane trafficking, and signaling–cytoskeleton crosstalk in human cells.
CAP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.