Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BTBD14B Double Nickase Plasmid (m): sc-426213-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BTBD14B Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BTBD14B Double Nickase Plasmid (m) and BTBD14B Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nacc1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BTBD14B Antibody (E-3): sc-376216
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BTBD14B Double Nickase Plasmid (m) | sc-426213-NIC | 20 µg | $410.00 | |||
BTBD14B Double Nickase Plasmid (m2) | sc-426213-NIC-2 | 20 µg | $410.00 |
Mouse Nacc1 encodes BTBD14B, a BTB/POZ domain–containing nuclear protein implicated in transcriptional regulation through protein–protein interactions and assembly of repressive or modulatory complexes. BTBD14B has been linked to control of cell state programs including proliferation, differentiation, and stress-responsive gene expression, consistent with roles in chromatin-associated regulation and ubiquitin-related protein turnover pathways. Dysregulation of Nacc1/BTBD14B has been associated in the literature with altered cell-cycle control and survival signaling, making it a useful node for studying context-dependent transcriptional networks in development and disease-relevant cellular phenotypes.
BTBD14B Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nacc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nacc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nacc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nacc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.