Bcl-xL Antibody (7B2.5): sc-56021

Bcl-xL Antibody (7B2.5) is a mouse monoclonal IgG3 antibody that detects Bcl-xL in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-Bcl-xL antibody (7B2.5) is available in a non-conjugated form, providing flexibility for various experimental setups. Bcl-xL, a critical regulator of apoptosis, plays a vital role in cell survival by inhibiting programmed cell death in response to various stressors. Bcl-xL (7B2.5) antibody targets a protein predominantly located in the outer mitochondrial membrane, where Bcl-xL functions to prevent the release of pro-apoptotic factors, maintaining cellular integrity and promoting cell survival. Bcl-xL′s presence at the mitochondria is essential for interaction with other key proteins involved in the apoptotic pathway, such as Bax and Bad. These interactions are crucial for the balance between cell survival and apoptosis, making Bcl-xL a significant player in cancer biology and therapeutic resistance. Understanding Bcl-xL dynamics and localization provides insights into potential therapeutic targets for enhancing apoptosis in cancer cells, thereby improving treatment outcomes.

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Bcl-xL Antibody (7B2.5) References:

1. Bcl-xL promotes metastasis independent of its anti-apoptotic activity.  |  Choi, S., et al. 2016. Nat Commun. 7: 10384. PMID: 26785948
2. BCL-XL binds and antagonizes RASSF6 tumor suppressor to suppress p53 expression.  |  Xu, X., et al. 2017. Genes Cells. 22: 993-1003. PMID: 29193479
3. BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells.  |  Afreen, S., et al. 2020. Cell Death Dis. 11: 8. PMID: 31907357
4. Bcl-xL inhibits PINK1/Parkin-dependent mitophagy by preventing mitochondrial Parkin accumulation.  |  Yu, S., et al. 2020. Int J Biochem Cell Biol. 122: 105720. PMID: 32088314
5. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells.  |  Loo, LSW., et al. 2020. Cell Death Dis. 11: 378. PMID: 32424151
6. Bcl-xL DNAzymes promote radiosensitivity and chemosensitivity in colorectal cancer cells via enhancing apoptosis.  |  Yu, Z., et al. 2022. BMC Pharmacol Toxicol. 23: 13. PMID: 35123593
7. Hydroxychloroquine Promotes Bcl-xL Inhibition-induced Apoptosis in BxPC-3 Human Pancreatic Cancer Cells.  |  Hoque, MM., et al. 2022. Anticancer Res. 42: 3495-3506. PMID: 35790256
8. Bcl-xL Promotes the Survival of Motor Neurons Derived from Neural Stem Cells.  |  Wu, Y., et al. 2023. Biology (Basel). 12: PMID: 36671824
9. Bcl-xL is required to protect endothelial cells latently infected with KSHV from virus induced intrinsic apoptosis.  |  Moore, LN., et al. 2023. PLoS Pathog. 19: e1011385. PMID: 37163552
10. Bcl-xL Is Spontaneously Inserted into Preassembled Nanodiscs and Stimulates Bax Insertion in a Cell-Free Protein Synthesis System.  |  Rouchidane Eyitayo, A., et al. 2023. Biomolecules. 13: PMID: 37371456

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bcl-x_L Antibody (7B2.5) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Bcl-x_L Antibody (7B2.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bcl-x_L Antibody (7B2.5) Specifications

Alternate Product Name: BCL2L1

Product Clone Name: 7B2.5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids:

Epitope Target: Bcl-x_L

Epitope Species: human

Product Reactivity/Detection: Bcl-xL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 50 µg IgG₃ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Bcl-x_L Antibody (7B2.5): N/A

The preferred Bcl-x_L antibody is Bcl-x_L Antibody (H-5): sc-8392.