BCECF/AM (CAS 117464-70-7)

用法 :

Cell staining for detecting cytoplasmic pH.

• Suspend cells in HEPES buffer solution (sc-300789) to prepare 4x10⁷ cells/ml.

• Add 1 mM BCECF-AM/DMSO solution to the cell suspension to prepare 3 uM BCECF-AM (1/300 vol of cell suspension) as the final concentration.

• Incubate the cell suspension at 37° C for 30 min.

• Wash the cells 3 times with HEPES buffer saline, and then prepare 3x106 cells/ml of the cell suspension.

•Determine the fluorescence intensity using a fluorescence microscope or a confocal laser microscope coupled with an image analyzer.

NOTE: Cell staining conditions differ by cell type, so it is necessary to optimize the conditions for each experiment.

身体の状態 :

Solid

溶解度 :

Soluble in acetone, methanol, DMSO, and DMF. Insoluble in water.

保存方法 :

Store at -20° C

沸騰点 :

637.07° C at 760 mmHg (Predicted)

密度 :

1.37 g/cm³ (Predicted)

屈折率 :

n²⁰_D 1.69 (Predicted)

IC50 :

diacylglycerol kinase in isolated platelet membrane: IC₅₀ = 300 nM; Inhibition of diacylglycerol kinase in intact platelets: IC₅₀ = 120 nM; mitochondrial import inner membrane translocase subunit TIM23: IC₅₀ = 5440 nM (Saccharomyces cerevisiae S288c)

免疫蛍光法 :

λex 505 nm, λem 520 nm in DMSO

pK値 :

pK_a: 11.24 (Predicted), pK_b: 8.33 (Predicted)

WGKドイツ :

3

MDL番号 :

MFCD00036969