B23/Nucleophosmin Antibody (7H10B9): sc-65943
- B23/Nucleophosmin Antibody (7H10B9) is a mouse monoclonal IgG1 provided at 100 µg/ml
- raised against B23 recombinant protein of human origin
- recommended for detection of B23 of human origin by WB, IP, IF and IHC(P)
- See B23/Nucleophosmin (E-3): sc-271737 for B23/Nucleophosmin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for B23/Nucleophosmin Antibody (7H10B9). This work is in progress.
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B23 Antibody (7H10B9) is a mouse monoclonal IgG1 antibody that detects B23 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. B23, also known as nucleophosmin, plays a crucial role in ribosomal assembly and the transport of ribosomal RNA, making B23 essential for protein synthesis and cellular function. B23 is predominantly located in the nucleolus, where B23 is involved in the maturation of ribosomal subunits, and proper localization is vital for maintaining efficient ribosome biogenesis. Additionally, B23 undergoes significant post-translational modifications, particularly phosphorylation by Cdc2 kinase during mitosis, which can influence B23′s activity and interactions with other cellular proteins. Anti-B23 antibody (7H10B9) serves as an invaluable tool for researchers studying ribosome assembly, cell cycle regulation, and nucleolar function, providing insights into the molecular mechanisms underlying cellular growth and proliferation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
B23/Nucleophosmin Antibody (7H10B9) References:
- Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone. | Okuwaki, M., et al. 2001. FEBS Lett. 506: 272-6. PMID: 11602260
- Nuclear Akt interacts with B23/NPM and protects it from proteolytic cleavage, enhancing cell survival. | Lee, SB., et al. 2008. Proc Natl Acad Sci U S A. 105: 16584-9. PMID: 18931307
- Involvement of nucleophosmin/B23 in the cellular response to curcumin. | Weng, CH., et al. 2011. J Nutr Biochem. 22: 46-52. PMID: 20303727
- Protein B23/nucleophosmin/numatrin nuclear dynamics in relation to protein kinase CK2 and apoptotic activity in prostate cells. | Wang, G., et al. 2010. Biochemistry. 49: 3842-52. PMID: 20387789
- The nucleolar phosphoprotein B23 targets Newcastle disease virus matrix protein to the nucleoli and facilitates viral replication. | Duan, Z., et al. 2014. Virology. 452-453: 212-22. PMID: 24606698
- Involvement of Nucleophosmin (NPM1/B23) in Assembly of Infectious HPV16 Capsids. | Day, PM., et al. 2015. Papillomavirus Res. 1: 74-89. PMID: 27398412
- Nucleophosmin/B23 (NPM) oligomer is a major and stable entity in HeLa cells. | Chan, PK. and Chan, FY. 1995. Biochim Biophys Acta. 1262: 37-42. PMID: 7772597
- Nucleolar protein B23 stimulates nuclear import of the HIV-1 Rev protein and NLS-conjugated albumin. | Szebeni, A., et al. 1997. Biochemistry. 36: 3941-9. PMID: 9092824
- Evidence for the ability of nucleophosmin/B23 to bind ATP. | Chang, JH., et al. 1998. Biochem J. 329 (Pt 3): 539-44. PMID: 9445380
- The nucleolar phosphoprotein B23 redistributes in part to the spindle poles during mitosis. | Zatsepina, OV., et al. 1999. J Cell Sci. 112 (Pt 4): 455-66. PMID: 9914158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
B23/Nucleophosmin Antibody (7H10B9) | sc-65943 | 100 µg/ml | $322.00 |