Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Double Nickase Plasmid (h): sc-400429-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Double Nickase Plasmid (h) and ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ASGR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Antibody (A-5): sc-393849
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Double Nickase Plasmid (h) | sc-400429-NIC | 20 µg | $410.00 | |||
ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Double Nickase Plasmid (h2) | sc-400429-NIC-2 | 20 µg | $410.00 |
ASGR1 encodes the major subunit of the hepatic asialoglycoprotein receptor (ASGPR1), a C-type lectin predominantly expressed on hepatocyte surfaces that recognizes desialylated glycoproteins bearing terminal galactose or N-acetylgalactosamine. Upon ligand binding, ASGPR1 mediates clathrin-dependent endocytosis and trafficking to endo-lysosomal compartments, supporting glycoprotein clearance and homeostatic regulation of circulating proteins. This receptor participates in carbohydrate recognition and vesicular transport processes that intersect with liver-specific metabolic pathways and receptor recycling dynamics. Altered ASGR1 function or expression is frequently investigated in contexts of hepatic dysfunction and lipid metabolism, making it a relevant target for mechanistic studies in liver biology.
ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ASGR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ASGR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ASGR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ASGR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.