Aminopeptidase P Antibody (JG12): sc-65390

Aminopeptidase P Antibody (JG12) is a mouse monoclonal IgG1 antibody that detects Aminopeptidase P protein of mouse and rat origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry. Anti-Aminopeptidase P antibody (JG12) is available as a non-conjugated format. Aminopeptidases, including Aminopeptidase P, play a crucial role in protein metabolism by catalyzing the removal of amino acids from the N-termini of proteins, which is essential for various physiological processes such as protein maturation and degradation. Aminopeptidase P (JG12) monoclonal antibody recognizes a member of the peptidase clan MG known for proline-specific activity, cleaving the N-terminal amino acid when the second residue is proline. Aminopeptidase P exists in two forms: a membrane-bound variant expressed on lymphoid cells, vascular endothelial cells in various tissues, and the brush-border membrane of kidney tubules and intestine, and a cytosolic form. The membrane-bound Aminopeptidase P is GPI-linked and consists of 674 amino acids, while the cytosolic form is 623 amino acids in length. Aminopeptidase P has been identified as a receptor for the breast-homing peptide, highlighting potential significance in developing targeted therapies for breast cancer.

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Aminopeptidase P Antibody (JG12) References:

1. Cloning, expression, and characterization of human cytosolic aminopeptidase P: a single manganese(II)-dependent enzyme.  |  Cottrell, GS., et al. 2000. Biochemistry. 39: 15121-8. PMID: 11106490
2. Molecular specialization of breast vasculature: a breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature.  |  Essler, M. and Ruoslahti, E. 2002. Proc Natl Acad Sci U S A. 99: 2252-7. PMID: 11854520
3. Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: insights into substrate recognition and the mechanism of catalysis.  |  Graham, SC., et al. 2006. Biochemistry. 45: 964-75. PMID: 16411772
4. Tyrosine 387 and arginine 404 are critical in the hydrolytic mechanism of Escherichia coli aminopeptidase P.  |  Jao, SC., et al. 2006. Biochemistry. 45: 1547-53. PMID: 16460001
5. Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli.  |  Király, O., et al. 2006. Protein Expr Purif. 48: 104-11. PMID: 16542853
6. Kinin-dependent hypersensitivity reactions in hemodialysis: metabolic and genetic factors.  |  Molinaro, G., et al. 2006. Kidney Int. 70: 1823-31. PMID: 17003818
7. Neuropeptide Y (NPY) cleaving enzymes: structural and functional homologues of dipeptidyl peptidase 4.  |  Frerker, N., et al. 2007. Peptides. 28: 257-68. PMID: 17223229
8. Live dynamic imaging of caveolae pumping targeted antibody rapidly and specifically across endothelium in the lung.  |  Oh, P., et al. 2007. Nat Biotechnol. 25: 327-37. PMID: 17334358
9. Purification, characterization, and cloning of a cytosolic aspartyl aminopeptidase.  |  Wilk, S., et al. 1998. J Biol Chem. 273: 15961-70. PMID: 9632644