ABC1 siRNA と shRNA プラスミド (h) human ABC1-スペシフィック siRNA、 shRNA プラスミドおよびレンチウイルス粒子遺伝子サイレンサー

ABC1 siRNA と shRNA プラスミド (h)

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データシート

    • 対象種: human; mouse対応製品につきまして、ABC1 siRNA and shRNA Plasmids (m)をチェックして下さい。
    • 遺伝子サイレンサーは一般的に3つから5つまでの標的特異的な長さ19-25ntのヌクレオチド配列のプールで構成されます。
    • ABC1遺伝子サイレンシングを独立的に検証できるよう、個々のsiRNAもリクエストに応じてご用意できます
    • 適したコントロール抗体: ABC1 抗体 (AC10): sc-53482 は免疫蛍光またはウエスタンブロットによってABC1発現ノックダウンのモニタリング用のコントロール抗体に推奨
    • si/shRNA 遺伝子サイレンサー の詳細につきましては、ここをクリックしてください。
    • 遺伝子サイレンサーは非営利と研究使用のみを目的としています。詳細はこちらをクリックしてください

Gene情報

動物種遺伝子名Gene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanABCA1199q31.1XM_005251773, XM_005251776, XM_011518339, XM_011518340, XM_011518341, XM_011518342, XM_011518344, XM_017014378, XM_017014379, XM_017014380, XM_017014381, XM_017014382, NM_005502O95477604091
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Does all sc lentivirus have the same promoters? The reason I ask is that I have seen others cited using sc lentivirus successfully in Thp-1 cells for LPL. I heard that promoter selection is crucial for Thp-1 cells.

質問者: weng
Thank you for your question. Yes, our vector uses an H1 promoter for shRNA transcription.
回答者: Technical Support
投稿日: 2019-04-23

I read the reference cited here. Is the shRNA targeting the same sequence? I want to make a stable knockdown of ABCA1 in Thp-1 cells. Does your shRNA guarantee knockdown? Does the product come with a positive control for transfection efficiency?

質問者: weng
Thank you for your question. Yes, the ABC1 shRNA plasmid (h): sc-41136-SH is using the same target sequence as the siRNA product that is referenced in the product citation on our website. For stable knockdown, you may want to consider using the shRNA Lentiviral Particles. Please contact our Technical Service Department for more information. We do sell separately a positive control for measuring transfection efficiency, copGFP Control Plasmid: sc-108083. https://www.scbt.com/scbt/browse/shrna-plasmid-support-products/_/N-lhyfam Santa Cruz does warranty these si/shRNA reagents to show observable knockdown at the protein level.  Santa Cruz implements computationally designed published algorithms which are demonstrated to give greater than 50% knockdown more than 90% of the time. If you purchase the reagents and are not able to achieve the expected results we encourage you to contact our technical support staff.  If we are unable to trouble-shoot the assay further then we are able to offer you either research credit or refund the purchase price of the reagents.
回答者: Technical Support
投稿日: 2019-04-23
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