2X Laemmli Sample Buffer For SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer.

2X Laemmli Sample Buffer

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アプリケーション: 2X Laemmli Sample Buffer is for SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer
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試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
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Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.


参考文献

1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Wu, M., et al. 2009. Diabetes Metab. Res. Rev. 25: 279-286. PMID: 19177471
3. Wu, M., et al. 2009. PLoS ONE. 4: e6430. PMID: 19641606

Formulation :
2X
Physical State :
Liquid
Solubility :
Soluble in water.
pH :
~6.8
保存方法 :
Store at -20° C
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
WGK Germany :
3
Transport :
UN 3082, Class 9, Packing group III

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2X Laemmli Sample Buffer  参考文献

See how others have used 2X Laemmli Sample Buffer. Click on the entry to view the PubMed entry .

Citations 1 to 5 of 5 total

PMID: # 27100161  Choh, V. et al. 2016. Investigative ophthalmology & visual science. 57: 2140-51.

PMID: # 26983346  Zhao, GX. et al. 2016. Biotech Histochem. 91: 269-76.

PMID: # 24629563  Yang, SJ. et al. 2014. Metab. Clin. Exp. 63: 693-701.

PMID: # 30292755  Biochem. Pharmacol. 158: 84-94.

PMID: # 15347737  Mol. Hum. Reprod. 705-11.

Citations 1 to 5 of 5 total
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