Ubp-M Antibody (B-3): sc-390683

Ubp-M Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ubp-M of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ubp-M (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ubiquitin-processing protease-M (Ubp-M) plays a crucial role in regulating protein degradation through its function as a deubiquitinating enzyme, which removes ubiquitin moieties from proteins tagged for degradation. This process is vital for maintaining cellular homeostasis, as it allows for protein recycling and regulation of various cellular pathways. Ubp-M is primarily localized in the cytosol and undergoes phosphorylation during the G2/M phase transition, which is essential for proper cell cycle progression. Ubp-M phosphorylation coincides with an increase in free ubiquitin chains and enhanced hydrolysis of ubiquitin-conjugated proteins, highlighting its role in modulating protein turnover dynamics. Ubp-M targets histone proteins H2A and H2B, which undergo monoubiquitination during interphase and anaphase, and deubiquitination during mitosis. This regulation of histone ubiquitination is critical for chromatin condensation during mitosis, indicating that Ubp-M influences both histone function and plays a significant role in orchestrating mitotic chromatin organization and cell growth progression.

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Ubp-M Antibody (B-3) References:

1. A mutant deubiquitinating enzyme (Ubp-M) associates with mitotic chromosomes and blocks cell division.  |  Cai, SY., et al. 1999. Proc Natl Acad Sci U S A. 96: 2828-33. PMID: 10077596
2. Isolation and characterization of protein A24, a 'histone-like' non-histone chromosomal protein.  |  Goldknopf, IL., et al. 1975. J Biol Chem. 250: 7182-7. PMID: 1165239
3. Metabolism of the polyubiquitin degradation signal: structure, mechanism, and role of isopeptidase T.  |  Wilkinson, KD., et al. 1995. Biochemistry. 34: 14535-46. PMID: 7578059
4. Ubiquitin, proteasomes, and the regulation of intracellular protein degradation.  |  Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
5. Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast.  |  Hecht, A., et al. 1995. Cell. 80: 583-92. PMID: 7867066
6. Pathways of ubiquitin conjugation.  |  Haas, AL. and Siepmann, TJ. 1997. FASEB J. 11: 1257-68. PMID: 9409544

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ubp-M Antibody (B-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Ubp-M Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ubp-M Antibody (B-3) Specifications

Alternate Product Name: Ubp-M

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 757-813

Epitope Target: Ubp-M

Epitope Species: human

Product Reactivity/Detection: Ubp-M

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Ubp-M Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin