Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Thyroperoxidase CRISPR/Cas9 KO Plasmid (m): sc-423484
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Thyroperoxidase CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Thyroperoxidase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Thyroperoxidase Antibody (A-5): sc-376876
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Thyroperoxidase CRISPR/Cas9 KO Plasmid (m) | sc-423484 | 20 µg | $397.00 |
Overview
Mouse Tpo encodes thyroperoxidase (TPO), a heme-dependent apical membrane enzyme in thyroid follicular cells that catalyzes iodide oxidation and the iodination and coupling of thyroglobulin to generate the thyroid hormones T3 and T4. TPO activity integrates iodide transport and organification with redox control via H2O2-producing systems such as DUOX/DUOXA, supporting hormone biosynthesis and thyroid follicle homeostasis. Disruption of Tpo-dependent thyroid hormonogenesis is linked to altered metabolic regulation, growth and neurodevelopmental phenotypes in model systems, and provides mechanistic entry points into autoimmune thyroid pathology where TPO is a major antigen. Accordingly, Tpo serves as a functional node for studying thyroid differentiation programs, endocrine feedback signaling, and oxidative stress responses in thyrocytes.
Thyroperoxidase CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tpo gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tpo together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tpo open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Thyroperoxidase protein expression.
This CRISPR knockout system enables efficient generation of Tpo-deficient cell models for investigation of Thyroperoxidase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tpo exon(s) critical for Thyroperoxidase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tpo genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Thyroperoxidase CRISPR/Cas9 KO Plasmid (m) and Thyroperoxidase CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tpo locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Thyroperoxidase HDR Plasmid (m) and Thyroperoxidase HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tpo homology arms to support homology-directed repair at defined Tpo target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.