TBZF CRISPR/Cas9 KO Plasmid (h): sc-417474
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TBZF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TBZF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TBZF CRISPR/Cas9 KO Plasmid (h) | sc-417474 | 20 µg | $397.00 |
Overview
ZNF675 encodes the human protein TBZF, a putative zinc finger DNA-binding factor implicated in transcriptional regulation and maintenance of cell-state–specific gene expression programs. As a likely nuclear regulator, TBZF is expected to influence chromatin-associated processes such as promoter/enhancer activity, transcriptional repression or activation, and coordination of downstream signaling-responsive gene networks. Dysregulation of zinc finger transcription factors is frequently linked to altered differentiation, proliferation, and stress-response pathways, making ZNF675 a relevant target for mechanistic studies of gene regulatory control in disease-associated cellular phenotypes. Investigating TBZF function can help clarify how transcriptional circuitry integrates with broader pathways governing genome stability and cellular homeostasis.
TBZF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZNF675 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZNF675 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZNF675 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TBZF protein expression.
This CRISPR knockout system enables efficient generation of ZNF675-deficient cell models for investigation of TBZF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZNF675 exon(s) critical for TBZF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZNF675 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TBZF CRISPR/Cas9 KO Plasmid (h) and TBZF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZNF675 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TBZF HDR Plasmid (h) and TBZF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZNF675 homology arms to support homology-directed repair at defined ZNF675 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.