Spt3 Antibody (D-4): sc-393650

Spt3 Antibody (D-4) is a mouse monoclonal IgG3 antibody that detects Spt3 in yeast (y) through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Spt3 plays a crucial role in the transcriptional regulation of RNA polymerase II-dependent genes, particularly as a member of the SAGA complex in Saccharomyces cerevisiae. Spt3 is essential for the recruitment of TATA-box binding protein (TBP) to core promoters, facilitating TBP binding to SAGA-dependent promoters, which is vital for proper gene expression. Notably, Spt3′s function is intricately linked to interactions with other proteins, such as Spt20 and GCN5; while Spt3 can inhibit TBP binding at certain promoters, GCN5 counteracts this repression through histone acetylation, promoting the formation of a TBP/TFIIA complex. The delicate balance of these interactions underscores the importance of Spt3 in regulating mating and developmental pathways in yeast, as null mutations in the Spt3 gene lead to significant defects in sporulation and growth patterns. With an ability to modulate transcriptional activity, Spt3 is a key player in the intricate network of gene regulation, making anti-Spt3 antibody (D-4) an invaluable tool for researchers studying these fundamental biological processes.

The preferred Spt3 antibody is Spt3 Antibody (D-11): sc-390356.