Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNX9 CRISPR/Cas9 KO Plasmid (m): sc-426112
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNX9 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SNX9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNX9 Antibody (G-5): sc-166863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNX9 CRISPR/Cas9 KO Plasmid (m) | sc-426112 | 20 µg | $397.00 |
Overview
Snx9 encodes sorting nexin 9 (SNX9), an SH3- and PX-domain adaptor that couples phosphoinositide binding to membrane remodeling and clathrin-mediated endocytosis. SNX9 coordinates assembly of endocytic and actin-regulatory machinery by interacting with dynamin and N-WASP, supporting vesicle scission, receptor internalization, and membrane trafficking at the plasma membrane. Through these roles, SNX9 influences signaling dynamics downstream of internalized receptors and contributes to cytoskeletal reorganization during processes such as cell migration and membrane turnover. Dysregulation of endocytic trafficking and actin control is implicated in cancer-related signaling and neurobiological phenotypes, making Snx9 a useful node for mechanistic studies of pathway rewiring in disease-relevant contexts.
SNX9 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Snx9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Snx9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Snx9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SNX9 protein expression.
This CRISPR knockout system enables efficient generation of Snx9-deficient cell models for investigation of SNX9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Snx9 exon(s) critical for SNX9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Snx9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SNX9 CRISPR/Cas9 KO Plasmid (m) and SNX9 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Snx9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SNX9 HDR Plasmid (m) and SNX9 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Snx9 homology arms to support homology-directed repair at defined Snx9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.