SKT CRISPR/Cas9 KO Plasmid (m): sc-431571
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SKT CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SKT genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SKT CRISPR/Cas9 KO Plasmid (m) | sc-431571 | 20 µg | $397.00 |
Overview
Mouse Etl4 encodes the SKT protein, a poorly characterized factor that is predicted to contribute to cell-intrinsic regulatory programs controlling gene expression and cellular homeostasis. Available annotations suggest involvement in fundamental processes such as proliferation, differentiation, and stress-adaptive responses, consistent with roles in developmental and tissue maintenance pathways. Because such regulators can influence pathway wiring and phenotypic stability, perturbation of SKT is useful for probing mechanisms that underlie disease-relevant cellular states, including dysregulated growth and altered lineage programs. Etl4/SKT loss-of-function models support mapping of downstream transcriptional and signaling consequences in mouse cells and in vivo contexts.
SKT CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Etl4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Etl4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Etl4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SKT protein expression.
This CRISPR knockout system enables efficient generation of Etl4-deficient cell models for investigation of SKT signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Etl4 exon(s) critical for SKT function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Etl4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SKT CRISPR/Cas9 KO Plasmid (m) and SKT CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Etl4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SKT HDR Plasmid (m) and SKT HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Etl4 homology arms to support homology-directed repair at defined Etl4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.