Rlf Double Nickase Plasmid (h): sc-410980-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rlf Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rlf Double Nickase Plasmid (h) and Rlf Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RLF. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rlf Double Nickase Plasmid (h) | sc-410980-NIC | 20 µg | $410.00 | |||
Rlf Double Nickase Plasmid (h2) | sc-410980-NIC-2 | 20 µg | $410.00 |
RLF (Rearranged L-myc fusion) encodes Rlf, a large nuclear protein implicated in transcriptional regulation and chromatin-associated processes through interactions with regulatory cofactors. Rlf has been linked to control of gene expression programs that influence cell-state maintenance, proliferation, and differentiation, consistent with roles in nuclear organization and epigenetic regulation. Altered RLF expression or locus disruption has been reported in genomic rearrangements in cancer, supporting its relevance for studying mechanisms of oncogenic transcriptional dysregulation. As a human gene with putative broad regulatory reach, RLF is frequently investigated to connect chromatin-dependent gene control to disease-associated phenotypes in cellular models.
Rlf Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RLF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RLF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RLF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RLF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.