PU.1/Spi1 Antibody (C-3): sc-390405

PU.1 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PU.1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PU.1 antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PU.1 plays a crucial role in hematopoiesis and is essential for the development and function of various immune cells, including B cells and myeloid cells. PU.1 acts as a transcription factor that regulates the expression of genes involved in immune responses, such as those encoding CD72, CD20, and Bruton′s tyrosine kinase (Btk). PU.1′s importance lies in its ability to influence lymphoid lineage commitment and differentiation, making PU.1 a key player in the immune system′s development and function. PU.1 interacts with other transcription factors, such as Spi-B, to enhance the expression of shared target genes, thereby coordinating the immune response. The Ets transcription factor family, to which PU.1 belongs, is characterized by its unique winged helix-turn-helix motif known as the Ets domain, which allows these proteins to bind to specific DNA sequences and regulate gene expression. Understanding PU.1′s function and interactions is vital for elucidating the mechanisms underlying immune cell development and the potential implications in immunological disorders.

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PU.1/Spi1 Antibody (C-3) References:

1. Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development.  |  Bemark, M., et al. 1999. J Biol Chem. 274: 10259-67. PMID: 10187812
2. PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction.  |  Garrett-Sinha, LA., et al. 1999. Immunity. 10: 399-408. PMID: 10229183
3. Precise developmental regulation of Ets family transcription factors during specification and commitment to the T cell lineage.  |  Anderson, MK., et al. 1999. Development. 126: 3131-48. PMID: 10375504
4. The Ets factors PU.1 and Spi-B regulate the transcription in vivo of P2Y10, a lymphoid restricted heptahelical receptor.  |  Rao, S., et al. 1999. J Biol Chem. 274: 34245-52. PMID: 10567398
5. Transcription factor PU.1 and immune cell differentiation (Review).  |  Li, G., et al. 2020. Int J Mol Med. 46: 1943-1950. PMID: 33125129
6. The role of a key transcription factor PU.1 in autoimmune diseases.  |  Fang, Y., et al. 2022. Front Immunol. 13: 1001201. PMID: 36248862
7. PU.1 is a useful nuclear marker to distinguish between histiocytosis and histiocyte-rich tumours.  |  Ungureanu, IA., et al. 2023. Histopathology. 83: 320-325. PMID: 37012662
8. PU.1 (Spi-1) autoregulates its expression in myeloid cells.  |  Chen, H., et al. 1995. Oncogene. 11: 1549-60. PMID: 7478579
9. The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation.  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
10. Neutrophils and monocytes express high levels of PU.1 (Spi-1) but not Spi-B.  |  Chen, HM., et al. 1995. Blood. 85: 2918-28. PMID: 7742552
11. The Ets protein Spi-B is expressed exclusively in B cells and T cells during development.  |  Su, GH., et al. 1996. J Exp Med. 184: 203-14. PMID: 8691135
12. PU.1: a crucial and versatile player in hematopoiesis and leukemia.  |  Kastner, P. and Chan, S. 2008. Int J Biochem Cell Biol. 40: 22-7. PMID: 17374502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PU.1/Spi1 Antibody (C-3) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-PU.1/Spi1 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PU.1/Spi1 Antibody (C-3) Specifications

Alternate Product Name: SPI1

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 25-63

Epitope Target: PU.1

Epitope Species: mouse

Product Reactivity/Detection: PU.1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for PU.1/Spi1 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent