Date published: 2026-5-7

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Pol II RPB5 Antibody (3Y11): sc-101605

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Datasheets
  • Pol II RPB5 Antibody (3Y11) is a mouse monoclonal IgG1, cited in 1 publications, provided at 100 µl ascites
  • raised against Pol II RPB5 of yeast origin
  • recommended for detection of Pol II RPB5 of yeast origin by WB
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Pol II RPB5 Antibody (3Y11). This work is in progress.
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Pol II RPB5 Antibody (3Y11) is a mouse monoclonal IgG1 antibody that detects Pol II RPB5 protein of yeast origin by western blotting (WB). Anti-Pol II RPB5 antibody (3Y11) is available in a non-conjugated form, specifically designed for researchers seeking to study the role of this essential protein in transcription. The POLR2E gene encodes RPB5 protein, which is a critical component of RNA polymerase II, responsible for synthesizing messenger RNA from DNA templates. RPB5 protein is located in the nucleus, where RPB5 plays a pivotal role in the transcription process, facilitating the assembly of the RNA polymerase complex and ensuring accurate gene expression. Proper functioning of RPB5 is vital, as RPB5 not only influences the transcription of protein-coding genes but also impacts various cellular processes, including cell growth and differentiation. Understanding the dynamics of RPB5 and interactions with other transcription factors can provide insights into the regulation of gene expression and the underlying mechanisms of various diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Pol II RPB5 Antibody (3Y11) References:

  1. Arabidopsis thaliana RNA polymerase II subunits related to yeast and human RPB5.  |  Larkin, RM., et al. 1999. Gene. 231: 41-7. PMID: 10231567
  2. Architecture of RNA polymerase II and implications for the transcription mechanism.  |  Cramer, P., et al. 2000. Science. 288: 640-9. PMID: 10784442
  3. Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target.  |  Todone, F., et al. 2000. Proc Natl Acad Sci U S A. 97: 6306-10. PMID: 10841537
  4. Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.  |  Kimura, M., et al. 2001. Eur J Biochem. 268: 612-9. PMID: 11168400
  5. Transcription organization and mRNA levels of the genes for all 12 subunits of the fission yeast RNA polymerase II.  |  Sakurai, H. and Ishihama, A. 2001. Genes Cells. 6: 25-36. PMID: 11168594
  6. Direct interaction between the subunit RAP30 of transcription factor IIF (TFIIF) and RNA polymerase subunit 5, which contributes to the association between TFIIF and RNA polymerase II.  |  Wei, W., et al. 2001. J Biol Chem. 276: 12266-73. PMID: 11278533
  7. Mutational analysis of human RNA polymerase II subunit 5 (RPB5): the residues critical for interactions with TFIIF subunit RAP30 and hepatitis B virus X protein.  |  Le, TT., et al. 2005. J Biochem. 138: 215-24. PMID: 16169872
  8. Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases.  |  Zaros, C., et al. 2007. Nucleic Acids Res. 35: 634-47. PMID: 17179178
  9. Mapping of Rpb3 and Rpb5 contact sites on two large subunits, Rpb1 and Rpb2, of the RNA polymerase II from fission yeast.  |  Miyao, T., et al. 1998. Mol Gen Genet. 259: 123-9. PMID: 9738888

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Pol II RPB5 Antibody (3Y11)

sc-101605
100 µl ascites
$339.00

What is the concentration of Pol II RPB5 (3Y11): sc-101605 monoclonal antibody?

Asked by: AbPolly
Thank you for your question. The concentration of Pol II RPB5 (3Y11): sc-101605 has not been characterized. We provide this antibody as 100 µl of ascites.
Answered by: Technical Support
Date published: 2017-03-24
  • y_2026, m_5, d_6, h_12CST
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Rated 5 out of 5 by from Good for Western BlotThis antibody can be used to detect yeast Pol II RPB5 by Western Blot. -SCBT QC
Date published: 2023-09-14
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Pol II RPB5 Antibody (3Y11) is rated 3.5 out of 5 by 2.
  • y_2026, m_5, d_6, h_12
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