PKD2 Antibody (F-2): sc-374344

PKD2 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects PKD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PKD2 Antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKD2 protein, also known as protein kinase D2, PRKD2, or HSPC187, is a crucial member of the protein kinase D family, which plays a significant role in various cellular processes. PKD2 is primarily located in the cytoplasm but can translocate to the nucleus upon activation, which is essential for regulating gene expression. This translocation is particularly important as PKD2 influences transcriptional activation of target genes, such as Nur77, while also promoting nuclear exclusion of histone deacetylase 7 (HDAC7). PKD2′s ability to shuttle between cytoplasm and nucleus underscores PKD2′s role in mediating signaling pathways initiated by G-protein-coupled receptors, thereby impacting processes like cell proliferation, apoptosis, and migration. anti-PKD2 antibody (F-2)′s diverse applications make this reagent an invaluable tool for researchers studying PKD2′s intricate roles in cellular signaling and gene regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PKD2 Antibody (F-2) References:

1. Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases.  |  Sturany, S., et al. 2001. J Biol Chem. 276: 3310-8. PMID: 11062248
2. Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells.  |  Irie, A., et al. 2006. Int Immunol. 18: 1737-47. PMID: 17077180
3. Immunohistochemical studies of protein kinase D (PKD) 2 expression in malignant human lymphomas.  |  Kovalevska, LM., et al. 2006. Exp Oncol. 28: 225-30. PMID: 17080017
4. Protein kinase D2 potentiates MEK/ERK/RSK signaling, c-Fos accumulation and DNA synthesis induced by bombesin in Swiss 3T3 cells.  |  Sinnett-Smith, J., et al. 2007. J Cell Physiol. 211: 781-90. PMID: 17226786
5. Angiotensin II directly triggers endothelial exocytosis via protein kinase C-dependent protein kinase D2 activation.  |  Ge, X., et al. 2007. J Pharmacol Sci. 105: 168-76. PMID: 17951978
6. Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2.  |  von Blume, J., et al. 2007. EMBO J. 26: 4619-33. PMID: 17962809
7. Protein kinase D2 regulates chromogranin A secretion in human BON neuroendocrine tumour cells.  |  von Wichert, G., et al. 2008. Cell Signal. 20: 925-34. PMID: 18262756
8. Expression patterns of protein kinase D 3 during mouse development.  |  Ellwanger, K., et al. 2008. BMC Dev Biol. 8: 47. PMID: 18439271
9. Human polycystin-2 transgene dose-dependently rescues ADPKD phenotypes in Pkd2 mutant mice.  |  Li, A., et al. 2015. Am J Pathol. 185: 2843-60. PMID: 26435415
10. Atypical calcium regulation of the PKD2-L1 polycystin ion channel.  |  DeCaen, PG., et al. 2016. Elife. 5: PMID: 27348301
11. Novel long-range regulatory mechanisms controlling PKD2 gene expression.  |  Moisan, S., et al. 2018. BMC Genomics. 19: 515. PMID: 29986647
12. BMP4 regulates asymmetric Pkd2 distribution in mouse nodal immotile cilia and ciliary mechanosensing required for left-right determination.  |  Katoh, TA., et al. 2024. Dev Dyn.. PMID: 38984461

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKD2 Antibody (F-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PKD2 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKD2 Antibody (F-2) Specifications

Alternate Product Name: PKD2

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 847-876

Epitope Target: PKD2

Epitope Species: human

Product Reactivity/Detection: PKD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PKD2 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin