p38 alpha/beta MAPK Antibody (A-12): sc-7972

p38 alpha/beta MAPK Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect p38 alpha (MAPK14) and p38 beta (MAPK11) proteins across multiple species, including mouse, rat, human, equine, and porcine. p38 alpha/beta MAPK mouse monoclonal antibody (A-12) recognizes an epitope within amino acids 213-360 at the C-terminus of human p38 alpha MAPK14. p38 MAPKs, also referred to as SAPK2A/SAPK2B or p38a/p38b, are vital members of the MAP kinase family that regulate essential cellular processes such as stress response, inflammation, cytokine production, and apoptosis. These proteins are integral to signal transduction pathways that control cell differentiation, proliferation, and survival, thereby maintaining cellular homeostasis and responding to environmental stimuli. p38 alpha/beta MAPK monoclonal antibody (A-12) is suitable for techniques including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA), making it a valuable tool for both research and diagnostic purposes. By targeting p38 MAPKs, p38 alpha/beta MAPK (A-12) antibody enables the investigation of critical cellular mechanisms and the underlying mechanisms of diseases like cancer, autoimmune disorders, and inflammatory conditions. p38 MAPKs depend on magnesium as a cofactor to facilitate the ATP-dependent phosphorylation of target proteins, and their activity is modulated through phosphorylation on threonine and tyrosine residues, which is essential for their function. p38 alpha/beta MAPK monoclonal antibody (A-12) is available in both non-conjugated and multiple conjugated forms, including agarose, HRP, PE, FITC, and several Alexa Fluor® conjugates, providing researchers with flexible options to meet diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p38 alpha/beta MAPK Antibody (A-12) References:

1. Requirement for p38alpha in erythropoietin expression: a role for stress kinases in erythropoiesis.  |  Tamura, K., et al. 2000. Cell. 102: 221-31. PMID: 10943842
2. Exip, a new alternative splicing variant of p38 alpha, can induce an earlier onset of apoptosis in HeLa cells.  |  Sudo, T., et al. 2002. Biochem Biophys Res Commun. 291: 838-43. PMID: 11866441
3. Cardiac expression and subcellular localization of the p38 mitogen-activated protein kinase member, stress-activated protein kinase-3 (SAPK3).  |  Court, NW., et al. 2002. J Mol Cell Cardiol. 34: 413-26. PMID: 11991731
4. Active mutants of the human p38alpha mitogen-activated protein kinase.  |  Diskin, R., et al. 2004. J Biol Chem. 279: 47040-9. PMID: 15284239
5. p38 and a p38-interacting protein are critical for downregulation of E-cadherin during mouse gastrulation.  |  Zohn, IE., et al. 2006. Cell. 125: 957-69. PMID: 16751104
6. Molecular cloning of human p38 MAP kinase.  |  Han, J., et al. 1995. Biochim Biophys Acta. 1265: 224-7. PMID: 7696354
7. A protein kinase involved in the regulation of inflammatory cytokine biosynthesis.  |  Lee, JC., et al. Nature. 372: 739-46. PMID: 7997261
8. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta).  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
9. The primary structure of p38 gamma: a new member of p38 group of MAP kinases.  |  Li, Z., et al. 1996. Biochem Biophys Res Commun. 228: 334-40. PMID: 8920915

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p38 alpha/beta MAPK Antibody (A-12) has 486 citations in a variety of scientific publications.

Primary antibodies like Anti-p38 alpha/beta MAPK Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p38 alpha/beta MAPK Antibody (A-12) Specifications

Alternate Product Name: p38 alpha/beta MAPK

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 213-360

Epitope Target: p38 alpha MAPK14

Epitope Species: human

Product Reactivity/Detection: p38 alpha MAPK14 and p38β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 486 citations in various scientific publications

Available Conjugates/Forms for p38 alpha/beta MAPK Antibody (A-12): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine