Anticorpo p22-phox (44.1) raccomandato per il rilevamento di p22-phox di origine mouse, rat e human in WB, IP e FCM

Anticorpo p22-phox (44.1): sc-130550

Anticorpo p22-phox (44.1) valutazione 4.5 di 5 di 4.
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Schede tecniche

    • p22-phox Antibody 44.1 è un monoclonale di topo IgG2a fornito in 200 µg/ml
    • raised against p22-phox of human origin, with epitope mapping to amino acids 29-33 and 182-188
    • p22-phox Antibody (44.1) é raccomandato per il rilevamento di p22-phox di origine mouse, rat e human in WB, IP e FCM
    • p22-phox Antibody (44.1) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
    • Contattaci per ricevere un FREE 10 µg sample di p22-phox (44.1): sc-130550.

Hello, You state that this clone can recognize mouse p22. Do you have any example immunoblot or flow cytometry data for this antibody on mouse cells? Thanks, David

Richiesta da: David M
Thank you for your question. We have not yet tested this mouse monoclonal antibody, p22-phox (44.1): sc-130550, in mouse or rat samples. However, the antibody is expected to cross-react with mouse and rat based on sequence identity with the antigen used to develop the antibody. We do warranty the antibody for detection of p22-phox of mouse, rat or human origin.
Risposta da: Technical Support
Data di pubblicazione: 2018-01-12

Can p22-phox (44.1): sc-130550 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Richiesta da: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, p22-phox (44.1): sc-130550 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Risposta da: Technical Support
Data di pubblicazione: 2017-02-24
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Rated 5 di 5 di da Perfect for FACS application We bought the sc-130550 PE (i.e. the PE coupled clone 44.1) and tested it on both PBMCs from a normal donor or from a p22phox mutated patient. The signal is absolutely perfect and specific since only the control shows a nice and strong peak.
Data di pubblicazione: 2017-04-06
Rated 4 di 5 di da Published WB data with human and hamster H661 Published WB data with human and hamster H661, CHO-91-47-67, CHO-K1 - SCBT Publication Review
Data di pubblicazione: 2015-01-26
Rated 5 di 5 di da Robust band present in western blot analysis Robust band present in western blot analysis of p22-phox expression in THP-1 whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-01-20
Rated 4 di 5 di da Great intracellular FCM data of fixed and Great intracellular FCM data of fixed and permeabilized THP-1 cells. -SCBT QC
Data di pubblicazione: 2013-12-04
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