Occludin Antibody (F-7): sc-271842

Occludin Antibody (F-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Occludin in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Occludin antibody (F-7) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Occludin is an integral membrane protein playing a crucial role in tight junction formation and maintenance within epithelial and endothelial cells, essential for regulating paracellular permeability and maintaining blood-brain barrier integrity. Occludin features a tetraspan structure with two extracellular loops facilitating interactions with other tight junction proteins. Post-translational modifications, particularly phosphorylation, significantly influence Occludin localization and functional properties. Phosphorylation of Occludin enhances transendothelial cell resistance and modulates cellular permeability to larger molecules. Expression levels of Occludin vary across tissues, with high levels in brain tissue at cell-cell contact sites, while non-neural tissues show lower and discontinuous expression patterns. Understanding Occludin expression and modification dynamics remains crucial for elucidating tight junction regulation and implications in various pathophysiological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Occludin Antibody (F-7) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-Occludin Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Occludin Antibody (F-7) Specifications

Alternate Product Name: Occludin

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2-27

Epitope Target: Occludin

Epitope Species: human

Product Reactivity/Detection: Occludin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for Occludin Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Occludin antibody is Occludin Antibody (E-5): sc-133256.