Nap-22 CRISPR/Cas9 KO Plasmid (h): sc-406361
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nap-22 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nap-22 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nap-22 CRISPR/Cas9 KO Plasmid (h) | sc-406361 | 20 µg | $397.00 |
Overview
BASP1 (Nap-22) is a membrane-associated, myristoylated protein enriched in neuronal and other excitable cells where it contributes to cortical actin organization, membrane dynamics, and phosphoinositide-dependent signaling. It modulates transcriptional programs through interactions with nuclear factors such as WT1 and participates in processes including neurite outgrowth, synaptic function, and cell differentiation. BASP1 activity has been linked to pathways governing cytoskeletal remodeling, signal transduction at the plasma membrane, and epigenetic control of gene expression. Altered BASP1 expression or regulation has been reported across multiple disease contexts, including neurodevelopmental phenotypes and cancer-associated changes in proliferation and invasion programs.
Nap-22 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BASP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BASP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BASP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nap-22 protein expression.
This CRISPR knockout system enables efficient generation of BASP1-deficient cell models for investigation of Nap-22 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BASP1 exon(s) critical for Nap-22 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BASP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nap-22 CRISPR/Cas9 KO Plasmid (h) and Nap-22 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BASP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nap-22 HDR Plasmid (h) and Nap-22 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BASP1 homology arms to support homology-directed repair at defined BASP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.