MKLP-1 Antibody (C-12): sc-390113

MKLP-1 (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects MKLP-1 protein (also designated mitotic kinesin-like protein 1, kinesin-like protein family member 6, kinesin-6, microtubule-dependent molecular motor, mitotic plus-end directed motor protein, anaphase centralspindlin complex, or KNSL5) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). MKLP-1 (C-12) antibody is available as non-conjugated form, as well as multiple conjugates including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. The monoclonal antibody CHO1 detects a spindle antigen required for mitotic progression. Screening a HeLa cell cDNA expression library with anti-MKLP-1 antibody (C-12) yields a cDNA encoding a protein related to motor ends of kinesin superfamily members within its amino terminal half. Since this similarity doesn′t extend further, CHO1 antigen, now designated MKLP-1, represents a novel kinesin. Sequence analysis predicts MKLP-1 possesses features typical of nuclear proteins. Immunocytological studies demonstrate MKLP-1 moves from the nucleus early in mitosis and then to the midbody after cytokinesis. MKLP-1 bundles antiparallel microtubules in vitro and moves them at rates comparable to spindle elongation in vivo. A hamster homolog of MKLP-1, designated CHO1 antigen, has also been isolated. Although functionally equivalent in microtubule bundling activity, significant differences exist between human and hamster proteins at their C-termini, possibly due to alternative splicing or multiple MKLP-1 loci.

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MKLP-1 Antibody (C-12) References:

1. Nuclear localization of C-terminal domains of the kinesin-like protein MKLP-1.  |  Deavours, BE. and Walker, RA. 1999. Biochem Biophys Res Commun. 260: 605-8. PMID: 10403813
2. A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles.  |  Nislow, C., et al. 1992. Nature. 359: 543-7. PMID: 1406973
3. Molecular interactions of Polo-like-kinase 1 with the mitotic kinesin-like protein CHO1/MKLP-1.  |  Liu, X., et al. 2004. J Cell Sci. 117: 3233-46. PMID: 15199097
4. Molecular components of the mitotic spindle.  |  Kuriyama, R. and Nislow, C. 1992. Bioessays. 14: 81-8. PMID: 1575716
5. MKLP1 requires specific domains for its dendritic targeting.  |  Xu, X., et al. 2006. J Cell Sci. 119: 452-8. PMID: 16418225
6. The nuclear localization signal of mitotic kinesin-like protein Mklp-1: effect on Mklp-1 function during cytokinesis.  |  Liu, X. and Erikson, RL. 2007. Biochem Biophys Res Commun. 353: 960-4. PMID: 17198681
7. A monoclonal antibody to a mitotic microtubule-associated protein blocks mitotic progression.  |  Nislow, C., et al. 1990. J Cell Biol. 111: 511-22. PMID: 2199459
8. Distribution of a matrix component of the midbody during the cell cycle in Chinese hamster ovary cells.  |  Sellitto, C. and Kuriyama, R. 1988. J Cell Biol. 106: 431-9. PMID: 2448315
9. Nuclear location signal-mediated protein transport.  |  Roberts, B. 1989. Biochim Biophys Acta. 1008: 263-80. PMID: 2667641
10. CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6.  |  Maruyama, Y., et al. 2021. Commun Biol. 4: 180. PMID: 33568771
11. Heterogeneity and microtubule interaction of the CHO1 antigen, a mitosis-specific kinesin-like protein. Analysis of subdomains expressed in insect sf9 cells.  |  Kuriyama, R., et al. 1994. J Cell Sci. 107 (Pt 12): 3485-99. PMID: 7706400
12. With apologies to scheherazade: tails of 1001 kinesin motors.  |  Goldstein, LS. 1993. Annu Rev Genet. 27: 319-51. PMID: 8122906

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MKLP-1 Antibody (C-12) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-MKLP-1 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MKLP-1 Antibody (C-12) Specifications

Alternate Product Name: Kinesin family member 23 (KIF23)

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-110

Epitope Target: MKLP-1

Epitope Species: human

Product Reactivity/Detection: MKLP-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for MKLP-1 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin