MALL CRISPR/Cas9 KO Plasmid (h): sc-406302
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MALL CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MALL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MALL CRISPR/Cas9 KO Plasmid (h) | sc-406302 | 20 µg | $397.00 |
Overview
MAL like (MALL) encodes a tetraspan membrane protein enriched in cholesterol- and sphingolipid-rich membrane microdomains, where it helps organize apical trafficking and membrane compartmentalization in epithelial cells. Through effects on lipid raft architecture, MALL can influence endocytic recycling, vesicular transport, and the spatial distribution of signaling complexes at the plasma membrane. Altered MALL expression has been observed across multiple tumor types and is studied in relation to epithelial differentiation state, cell polarity, and microdomain-dependent signaling behavior. These properties make MALL relevant for investigating membrane organization mechanisms that modulate proliferation, adhesion, and receptor dynamics in human cellular models.
MALL CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MALL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MALL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MALL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MALL protein expression.
This CRISPR knockout system enables efficient generation of MALL-deficient cell models for investigation of MALL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MALL exon(s) critical for MALL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MALL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MALL CRISPR/Cas9 KO Plasmid (h) and MALL CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MALL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MALL HDR Plasmid (h) and MALL HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MALL homology arms to support homology-directed repair at defined MALL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.