Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAfF Double Nickase Plasmid (h): sc-411785-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAfF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAfF Double Nickase Plasmid (h) and MAfF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAFF. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAfF Double Nickase Plasmid (h) | sc-411785-NIC | 20 µg | $410.00 | |||
MAfF Double Nickase Plasmid (h2) | sc-411785-NIC-2 | 20 µg | $410.00 |
MAFF encodes MAfF, a small Maf basic leucine zipper transcription factor that lacks a canonical transactivation domain and typically functions as an obligate dimer partner for CNC and other bZIP proteins. Through heterodimerization with factors such as NFE2L2/NRF2, MAfF contributes to regulation of antioxidant response element (ARE)-driven transcription and integrates cellular programs controlling redox homeostasis, xenobiotic metabolism, and inflammatory signaling. MAfF activity is therefore linked to oxidative stress adaptation and transcriptional reprogramming in contexts including metabolic dysregulation and tumor biology. Altered MAFF expression has been reported in multiple disease-relevant settings, supporting its utility as a node for dissecting stress-response networks and downstream gene regulatory circuits.
MAfF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAFF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAFF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAFF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAFF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.