MAD1 Antibody (117-468): sc-65494

MAD1 Antibody (117-468) is a mouse monoclonal IgG1 antibody that detects MAD1 in human samples through applications such as immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD1 plays a critical role in the regulation of the cell cycle, particularly at the mitotic spindle assembly checkpoint, where MAD1 ensures all chromosomes are properly aligned before the cell proceeds to anaphase. This function is vital for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy and contribute to cancer development. MAD1 is primarily localized in the nucleus, but MAD1′s dynamic movement during mitosis to the centrosome, spindle midzone, and midbody highlights MAD1′s importance in coordinating the cell division process. MAD1 interacts with MAD2 to form a tetrameric core complex, which is essential for checkpoint function. MAD1′s activity is regulated by phosphorylation, particularly in response to spindle damage, underscoring MAD1′s role as a key player in the cellular response to mitotic stress. Defects in the gene encoding MAD1, known as MAD1L1, have been implicated in various cancer types, making MAD1 a significant target for research and therapeutic strategies.

The preferred MAD1 antibody is MAD1 Antibody (9B10): sc-47746.