LBX1 CRISPR/Cas9 KO Plasmid (h): sc-406846
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LBX1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LBX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LBX1 CRISPR/Cas9 KO Plasmid (h) | sc-406846 | 20 µg | $397.00 |
Overview
LBX1 (ladybird homeobox 1) encodes a homeobox transcription factor that regulates embryonic patterning and lineage specification, with prominent roles in spinal cord interneuron differentiation and skeletal muscle precursor migration. Through sequence-specific DNA binding, LBX1 helps coordinate transcriptional programs that shape dorsoventral neural tube development and myogenic pathways during somitogenesis. Genetic variation and altered regulation of LBX1 have been associated with developmental phenotypes, including susceptibility to adolescent idiopathic scoliosis, highlighting its relevance to musculoskeletal and neurodevelopmental research. In cellular models, LBX1 is commonly studied for its impact on differentiation state, cell identity maintenance, and downstream transcriptional networks.
LBX1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LBX1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LBX1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LBX1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LBX1 protein expression.
This CRISPR knockout system enables efficient generation of LBX1-deficient cell models for investigation of LBX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LBX1 exon(s) critical for LBX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LBX1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LBX1 CRISPR/Cas9 KO Plasmid (h) and LBX1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LBX1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LBX1 HDR Plasmid (h) and LBX1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LBX1 homology arms to support homology-directed repair at defined LBX1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.