IP3KB Antibody (J-15): sc-100385

IP3KB Antibody (J-15) is a mouse monoclonal IgG2b kappa light chain antibody that detects IP3KB protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-IP3KB antibody (J-15) is available as a non-conjugated format. IP3KB protein plays a crucial role in cellular signaling by regulating intracellular calcium levels through action on inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), which releases calcium from internal stores. This process is vital for various physiological functions, including muscle contraction, neurotransmitter release, and cell proliferation. The phosphorylation of Ins(1,4,5)P3 by inositol 1,4,5-trisphosphate 3-kinase (IP3K) leads to the formation of inositol 1,3,4,5-tetrakisphosphate (Ins(1,4,5)P4), which is believed to further regulate calcium influx across the plasma membrane. IP3K exists in three isoforms—IP3KA, B, and C—with IP3KB being expressed in multiple rat tissues, including the lung, thymus, testis, brain, and heart. IP3K activity is enhanced in the presence of calmodulin and is modulated by phosphorylation through various kinases, thereby influencing the inositol phosphate signaling pathways that are essential for maintaining cellular homeostasis and responding to external stimuli.

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IP3KB Antibody (J-15) References:

1. Identification of the actin-binding domain of Ins(1,4,5)P3 3-kinase isoform B (IP3K-B).  |  Brehm, MA., et al. 2004. Biochem J. 382: 353-62. PMID: 15130091
2. Inositol tetrakisphosphate as a second messenger: confusions, contradictions, and a potential resolution.  |  Irvine, RF. 1991. Bioessays. 13: 419-27. PMID: 1659392
3. Inositol trisphosphate 3-kinase B (InsP3KB) as a physiological modulator of myelopoiesis.  |  Jia, Y., et al. 2008. Proc Natl Acad Sci U S A. 105: 4739-44. PMID: 18339802
4. Human inositol 1,4,5-trisphosphate 3-kinase isoform B (IP3KB) is a nucleocytoplasmic shuttling protein specifically enriched at cortical actin filaments and at invaginations of the nuclear envelope.  |  Nalaskowski, MM., et al. 2011. J Biol Chem. 286: 4500-10. PMID: 21148483
5. Regulation of D-myo-inositol 1,4,5-trisphosphate 3-kinase by cAMP-dependent protein kinase and protein kinase C.  |  Sim, SS., et al. 1990. J Biol Chem. 265: 10367-72. PMID: 2162347
6. Cloning and expression in Escherichia coli of a rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase.  |  Takazawa, K., et al. 1990. Biochem J. 272: 107-12. PMID: 2176078
7. Inositol trisphosphate 3-kinase B is increased in human Alzheimer brain and exacerbates mouse Alzheimer pathology.  |  Stygelbout, V., et al. 2014. Brain. 137: 537-52. PMID: 24401760
8. Inositol phosphates and cell signalling.  |  Berridge, MJ. and Irvine, RF. 1989. Nature. 341: 197-205. PMID: 2550825
9. Purification of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain.  |  Johanson, RA., et al. 1988. J Biol Chem. 263: 7465-71. PMID: 2836386
10. Severe combined immunodeficiency caused by inositol-trisphosphate 3-kinase B (ITPKB) deficiency.  |  Almutairi, A., et al. 2020. J Allergy Clin Immunol. 145: 1696-1699.e6. PMID: 31987846
11. Tissue- and cell-specific expression of Ins(1,4,5)P3 3-kinase isoenzymes.  |  Vanweyenberg, V., et al. 1995. Biochem J. 306 (Pt 2): 429-35. PMID: 7887896
12. Expression, purification, and regulation of two isoforms of the inositol 1,4,5-trisphosphate 3-kinase.  |  Woodring, PJ. and Garrison, JC. 1997. J Biol Chem. 272: 30447-54. PMID: 9374536

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IP3KB Antibody (J-15) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-IP3KB Antibody (J-15) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IP3KB Antibody (J-15) Specifications

Alternate Product Name: IP3KB

Product Clone Name: J-15

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 545-644

Epitope Target: IP3KB

Epitope Species: human

Product Reactivity/Detection: IP3KB

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for IP3KB Antibody (J-15): N/A