HNF-3β CRISPR/Cas9 KO Plasmid (m): sc-420890

Overview

Foxa2 encodes the forkhead box transcription factor HNF-3β, a pioneer factor that binds compacted chromatin to establish and maintain endodermal gene regulatory programs. In mouse, HNF-3β coordinates transcriptional networks controlling foregut and notochord development, hepatopancreatic lineage specification, and epithelial differentiation, integrating signals from pathways such as Wnt, TGF-β/SMAD, Hedgehog, and nuclear receptor–mediated metabolism. In adult tissues, FOXA2 influences glucose and lipid homeostasis by regulating hepatic metabolic genes and modulating endocrine and epithelial cell identity. Dysregulated FOXA2 activity has been linked to developmental defects and to disease-relevant states involving altered differentiation, epithelial plasticity, and metabolic dysfunction, supporting its use in mechanistic studies of transcriptional control.

HNF-3β CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Foxa2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Foxa2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Foxa2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HNF-3β protein expression.

This CRISPR knockout system enables efficient generation of Foxa2-deficient cell models for investigation of HNF-3β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Foxa2 exon(s) critical for HNF-3β function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Foxa2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by HNF-3β CRISPR/Cas9 KO Plasmid (m) and HNF-3β CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Foxa2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by HNF-3β HDR Plasmid (m) and HNF-3β HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Foxa2 homology arms to support homology-directed repair at defined Foxa2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.