Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GFRα-3 CRISPR/Cas9 KO Plasmid (h): sc-405061
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GFRα-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GFRα-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GFRα-3 Antibody (C-3): sc-398618
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GFRα-3 CRISPR/Cas9 KO Plasmid (h) | sc-405061 | 20 µg | $397.00 |
Overview
GFRA3 encodes the human glial cell line–derived neurotrophic factor (GDNF) family receptor alpha-3 (GFRα-3), a GPI-anchored co-receptor that confers ligand specificity for the neurotrophic factor artemin and related GDNF family ligands. Upon ligand binding, GFRα-3 cooperates with the RET receptor tyrosine kinase to initiate signaling cascades including MAPK/ERK and PI3K/AKT that regulate neuronal survival, differentiation, and axonal guidance. GFRA3 is broadly studied in peripheral nervous system development and neuro-immune interactions, where altered receptor-ligand signaling has been linked to mechanisms relevant to sensory neuron function and inflammatory pain processing. Dysregulated RET/GDNF-family signaling involving GFRA3 is also investigated in contexts of tumor cell signaling, invasion, and microenvironmental crosstalk in select cancer models.
GFRα-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GFRA3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GFRA3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GFRA3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GFRα-3 protein expression.
This CRISPR knockout system enables efficient generation of GFRA3-deficient cell models for investigation of GFRα-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GFRA3 exon(s) critical for GFRα-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GFRA3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GFRα-3 CRISPR/Cas9 KO Plasmid (h) and GFRα-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GFRA3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GFRα-3 HDR Plasmid (h) and GFRα-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GFRA3 homology arms to support homology-directed repair at defined GFRA3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.