Anti-FBXO29 Antibody D-7 è un monoclonale di topo IgG1 (kappa light chain) FBXO29 antibody fornito in 200 µg/ml
generato contro gli amminoacidi 284-403 mappati nella regione interna di FBXO29 di origine mouse
FBXO29 Antibody (D-7) é raccomandato per il rilevamento di FBXO29 di origine mouse, rat e human in WB, IP, IF e ELISA
FBXO29 Antibody (D-7) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
Contattaci per ricevere un FREE 10 µg sample di FBXO29 (D-7): sc-514385.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for FBXO29 Antibody (D-7) for WB applications. This reagent is now offered in a bundle with FBXO29 Antibody (D-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
FBXO29 Antibody (D-7) is a high quality monoclonal FBXO29 antibody (also designated FBXW8 antibody) suitable for the detection of the FBXO29 protein of mouse, rat and human origin. FBXO29 Antibody (D-7) is available as both the non-conjugated anti-FBXO29 antibody form, as well as multiple conjugated forms of anti-FBXO29 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. FBXO29 (F-box only protein 29), also designated F-box/WD repeat-containing protein 8 (FBXW8), is a 598 amino acid protein that contains one forty amino acid F-box region, making it a member of the F-box family. FBXO29 also contains five WD repeats. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. F-box proteins are members of a large family that regulates cell cycle, immune response, signaling cascades and developmental programs by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-α and β-catenin, for degradation by the proteasome after ubiquitination. Functioning as a component of the SCF complex, FBXO29 is thought to recognize and bind to select phosphorylated proteins, thereby promoting their ubiquitination and subsequent degradation. FBXO29 exists as two isoforms as a result of alternative splicing events.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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What is the recommended fixation method for immunofluorescence staining with FBXO29 (D-7): sc-514385 monoclonal antibody?
Richiesta da: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
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