eIF4G Antibody (H-2): sc-373892
- eIF4G Antibody (H-2) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1339-1372 near the C-terminus of eIF4G of human origin
- recommended for detection of eIF4G of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, bovine and porcine
- also available conjugated to biotin for WB, IHC(P) and ELISA
- See eIF4G (A-10): sc-133155 for eIF4G antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eIF4G Antibody (H-2) for WB applications. These reagents are now offered in bundles with eIF4G Antibody (H-2) (see ordering information below).
eIF4G Antibody (H-2) is a mouse monoclonal IgG2a antibody that detects eIF4G in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eIF4G plays a crucial role in the initiation of protein synthesis by serving as a scaffold that links the mRNA cap-binding protein eIF4E and the RNA helicase eIF4A, facilitating the assembly of the eukaryotic initiation complex. This complex is essential for the recruitment of ribosomes to mRNA, allowing for the unwinding of secondary structures in the mRNA that could impede translation. The proper functioning of eIF4G is vital for regulating gene expression and ensuring that proteins are synthesized efficiently, which is particularly important in processes such as cell growth, differentiation, and response to stress. Dysregulation of eIF4G has been implicated in various diseases, including cancer, making eIF4G a significant target for research and therapeutic interventions. Anti-eIF4G antibody (H-2) provides a reliable tool for studying the dynamics of protein synthesis and the role of eIF4G in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF4G Antibody (H-2) References:
- The translation of capped mRNAs has an absolute requirement for the central domain of eIF4G but not for the cap-binding initiation factor eIF4E. | Ali, IK. and Jackson, RJ. 2001. Cold Spring Harb Symp Quant Biol. 66: 377-87. PMID: 12762040
- eIF4G is required for the pioneer round of translation in mammalian cells. | Lejeune, F., et al. 2004. Nat Struct Mol Biol. 11: 992-1000. PMID: 15361857
- eIF4G stimulates the activity of the DEAD box protein eIF4A by a conformational guidance mechanism. | Hilbert, M., et al. 2011. Nucleic Acids Res. 39: 2260-70. PMID: 21062831
- Synergistic activation of eIF4A by eIF4B and eIF4G. | Nielsen, KH., et al. 2011. Nucleic Acids Res. 39: 2678-89. PMID: 21113024
- The Structures of eIF4E-eIF4G Complexes Reveal an Extended Interface to Regulate Translation Initiation. | Grüner, S., et al. 2016. Mol Cell. 64: 467-479. PMID: 27773676
- Structural motifs in eIF4G and 4E-BPs modulate their binding to eIF4E to regulate translation initiation in yeast. | Grüner, S., et al. 2018. Nucleic Acids Res. 46: 6893-6908. PMID: 30053226
- Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells. | Frosi, Y., et al. 2020. J Vis Exp.. PMID: 32421011
- RNA-tethering assay and eIF4G:eIF4A obligate dimer design uncovers multiple eIF4F functional complexes. | Robert, F., et al. 2020. Nucleic Acids Res. 48: 8562-8575. PMID: 32749456
- The domains of yeast eIF4G, eIF4E and the cap fine-tune eIF4A activities through an intricate network of stimulatory and inhibitory effects. | Krause, L., et al. 2022. Nucleic Acids Res. 50: 6497-6510. PMID: 35689631
- The eIF4G-eIF4E complex is the target for direct cleavage by the rhinovirus 2A proteinase. | Haghighat, A., et al. 1996. J Virol. 70: 8444-50. PMID: 8970966
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4G Antibody (H-2) | sc-373892 | 200 µg/ml | $322.00 | |||
eIF4G Antibody (H-2): m-IgGκ BP-HRP Bundle | sc-535331 | 200 µg Ab; 40 µg BP | $361.00 | |||
eIF4G Antibody (H-2): m-IgG2a BP-HRP Bundle | sc-546222 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF4G Antibody (H-2) B | sc-373892 B | 200 µg/ml | $320.00 | |||
eIF4G (H-2) Neutralizing Peptide | sc-373892 P | 100 µg/0.5 ml | $69.00 |