
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynactin 1 CRISPR/Cas9 KO Plasmid (h) | sc-402369 | 20 µg | $397.00 |
DCTN1 encodes dynactin 1, the major p150Glued subunit of the dynactin complex that couples cytoplasmic dynein to cargo and microtubules to support processive minus-end–directed transport. Dynactin 1 contributes to vesicle and organelle trafficking, endosome–lysosome positioning, Golgi organization, mitotic spindle function, and neuronal axonal transport through dynein–dynactin motor regulation. By coordinating transport and cytoskeletal dynamics, DCTN1 influences pathways underlying proteostasis, autophagic flux, and stress granule handling in long-lived cells such as neurons. Genetic disruption or dysfunction of DCTN1 has been linked to neurodegenerative phenotypes, making it a relevant target for mechanistic studies of motor-driven transport defects and neuronal vulnerability.
Dynactin 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCTN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCTN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCTN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dynactin 1 protein expression.
This CRISPR knockout system enables efficient generation of DCTN1-deficient cell models for investigation of Dynactin 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
CRISPRs +/- HDRs
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.