Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dynactin 1 CRISPR/Cas9 KO Plasmid (h): sc-402369
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dynactin 1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dynactin 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dynactin 1 Antibody (A-2): sc-365274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dynactin 1 CRISPR/Cas9 KO Plasmid (h) | sc-402369 | 20 µg | $397.00 |
Overview
DCTN1 encodes dynactin 1, the major p150Glued subunit of the dynactin complex that couples cytoplasmic dynein to cargo and microtubules to support processive minus-end–directed transport. Dynactin 1 contributes to vesicle and organelle trafficking, endosome–lysosome positioning, Golgi organization, mitotic spindle function, and neuronal axonal transport through dynein–dynactin motor regulation. By coordinating transport and cytoskeletal dynamics, DCTN1 influences pathways underlying proteostasis, autophagic flux, and stress granule handling in long-lived cells such as neurons. Genetic disruption or dysfunction of DCTN1 has been linked to neurodegenerative phenotypes, making it a relevant target for mechanistic studies of motor-driven transport defects and neuronal vulnerability.
Dynactin 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DCTN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DCTN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DCTN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dynactin 1 protein expression.
This CRISPR knockout system enables efficient generation of DCTN1-deficient cell models for investigation of Dynactin 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DCTN1 exon(s) critical for Dynactin 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DCTN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dynactin 1 CRISPR/Cas9 KO Plasmid (h) and Dynactin 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DCTN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dynactin 1 HDR Plasmid (h) and Dynactin 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DCTN1 homology arms to support homology-directed repair at defined DCTN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.