Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRM1 CRISPR/Cas9 KO Plasmid (h2): sc-400348-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRM1 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRM1 Antibody (C-1): sc-74454
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRM1 CRISPR/Cas9 KO Plasmid (h2) | sc-400348-KO-2 | 20 µg | $397.00 |
Overview
XPO1 encodes the nuclear export receptor CRM1, a karyopherin-β family protein that recognizes leucine-rich nuclear export signals and mediates RanGTP-dependent transport of proteins and select RNAs from the nucleus to the cytoplasm. By controlling nucleocytoplasmic trafficking, CRM1 regulates cell-cycle progression, stress responses, and transcriptional programs through localization of cargos such as tumor suppressors, transcription factors, and ribonucleoprotein complexes. CRM1 function integrates with the Ran GTPase cycle and nuclear pore complex dynamics to maintain compartmentalized signaling and proteostasis. Dysregulated XPO1/CRM1 activity and altered cargo distribution are frequently studied in cancer biology, antiviral responses, and neurodegenerative mechanisms where nuclear–cytoplasmic transport is perturbed.
CRM1 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the XPO1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the XPO1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the XPO1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRM1 protein expression.
This CRISPR knockout system enables efficient generation of XPO1-deficient cell models for investigation of CRM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting XPO1 exon(s) critical for CRM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple XPO1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRM1 CRISPR/Cas9 KO Plasmid (h) and CRM1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the XPO1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRM1 HDR Plasmid (h) and CRM1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by XPO1 homology arms to support homology-directed repair at defined XPO1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.