COL4A2 CRISPR/Cas9 KO Plasmid (m): sc-419744
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL4A2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL4A2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL4A2 CRISPR/Cas9 KO Plasmid (m) | sc-419744 | 20 µg | $397.00 |
Overview
Col4a2 encodes the α2 chain of type IV collagen (COL4A2), a core structural component of basement membranes that forms a heterotrimeric network with other collagen IV chains to support tissue architecture. This extracellular matrix scaffold regulates cell adhesion, migration, and polarity and shapes signaling crosstalk with integrins and growth factor pathways involved in vascular stability and organ development. Altered COL4A2 function is linked to basement membrane fragility and small-vessel pathology, making it relevant to studies of cerebrovascular integrity, ocular development, and kidney filtration barriers. In mouse models, Col4a2 provides a tractable entry point for dissecting matrix-driven mechanisms that influence endothelial and epithelial behavior.
COL4A2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Col4a2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Col4a2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Col4a2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL4A2 protein expression.
This CRISPR knockout system enables efficient generation of Col4a2-deficient cell models for investigation of COL4A2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Col4a2 exon(s) critical for COL4A2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Col4a2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL4A2 CRISPR/Cas9 KO Plasmid (m) and COL4A2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Col4a2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL4A2 HDR Plasmid (m) and COL4A2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Col4a2 homology arms to support homology-directed repair at defined Col4a2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.